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The recycling of traditional Chinese medicine （TCM） wastes is an important research topic to be solved urgently in the industrialization of TCM resources. Rhei Radix et Rhizoma is a bulk Chinese herb mainly derived from Rheum palmatum，R. tanguticum，and R. officinale. At present，these three medicinal plants have been cultivated on a large scale and widely used in the fields of medicine，health care，food，cosmetics，and veterinary medicine，with an annual demand of more than 5 500 tons（1 ton=1 000 kg）. However，a large number of wastes such as non-medicinal parts and residues produced in the production and deep processing are discarded because there is no effective way of utilization，resulting in serious waste of resources and environmental pollution. The non-medicinal parts contain not only the chemical components and pharmacological effects similar to those of roots and rhizomes but also a variety of amino acids，mineral elements，and conventional nutrients. They have a long history of use，and the content of some resource components is higher than that in roots and rhizomes. In particular，their stems and leaves exhibit great potential to be consumed as food and medicine due to high safety. Besides，the content of anthraquinones in Rhei Radix et Rhizoma residue is high and it possesses good antibacterial activity. It can be seen that the waste from the industrialization of Rhei Radix et Rhizoma has high utilization value. Hence，based on the relevant literature and investigation on the application of producing areas in China and abroad，the paper summarized the utilization status of their medicinal and non-medicinal parts，the waste production in the industrialization，as well as the active substances and utilization ways and put forward the multi-level and multi-path utilization strategy of Rhei Radix et Rhizoma wastes，in order to provide reference for the rational development and application of Rhei Radix et Rhizoma resources and promote the effective utilization and green development of the corresponding wastes.
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Objective To propose a method for analyzing the hip joint signals during human walking based on Hilbert-Huang transform (HHT) method and verify its feasibility. Methods First, the hip joint angles of one healthy subject were measured by using the hip joint measuring platform composed of acceleration sensors and gyroscopes. Second, all intrinsic mode functions (IMFs) at different scales, which could be further analyzed and combined, were obtained by applying the ensemble empirical mode decomposition (EEMD) to original signals. Finally, the Hilbert spectrum of original signals were plotted and analyzed. Results The signals representing different motion modes as well as gait characteristics indicated by rotating track of the hip joint were obtained. The Hilbert spectrum could show the intra-wave frequency modulation in the main motion mode and the characteristics of walking frequencies. Conclusions This method can be used in rehabilitation and treatment of patients with gait diseases. By using this method, the characteristic signals of the hip joints at different frequency scales can be effectively decomposed, and the post-processing signals can be filtered and centrally corrected, so as to adaptively analyze gait signals of the patients.
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Objective To accurately measure the motion angels of hand-related joints during manipulations of acupuncture needle thrusting-pulling and twirling, so as to provide quantitative references for acupuncture manipulation. Methods Six acupuncturists with over 3-year acupuncture experience and one volunteer were enrolled in this study. The angles of the forearm, wrist, metacarpophalangeal & interphalangeal joints of the thumb and index finger in each acupuncturist when performing thrusting-pulling and twirling manipulation were measured by the video motion capture (VMC) system, and the different ranges of above-mentioned angles among the 6 acupuncturists when performing 10 trails of thrusting-pulling and twirling manipulations on the volunteer’s thigh were compared. Results There was no significant difference in the ranges of relevant hand-related angles in the acupuncturist (P>0.05). The manipulation of thrusting-pulling was mainly managed by the wrist joint, with the range of (7.23±1.87)°, while the manipulation of twirling was mainly managed by the interphalangeal joints of the index finger, and the range of the first and second interphalangeal joints of the index finger was (28.33±2.18)°and (10.43±1.69)°, respectively. Conclusions The VMC can be a reliable method to quantify the parameters of acupuncture manipulation. Different acupuncture manipulation shows particular variation of the joint angles, which can be used as a reference to quantify the acupuncture manipulation.
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Objective To establish a neural probe-brain tissue numerical model and investigate tissue injuries induced by probe during its insertion into brain tissues. Methods The material of brain tissue was described by a hyper-viscoelastic constitutive equation. Tissue failure and separation were simulated by the element deletion method based on a maximum principle strain failure criteria, and tissue injuries were quantified by the mean effective strain. Then effects of probe wedge angle, inserting speed and probe stiffness on the acute injury were investigated. Results Tissue strain generated by probe with wedge angle of 150° was increased by 37.1% compared with the strain induced with wedge angle of 90°. Along the insertion path, probe with a slow speed of 100 μm/s induced much higher strain value (>57%) compared to that with relatively faster speed of 500 μm/s, which generated the strain value below 25%. The probe stiffness, however, had a negligible effect on tissue injury. The strain within the tissue was only increased by 1%-2% while the stiffness decreased from 165 GPa to 5 kPa. Conclusions The established numerical model can provide references for the design of neural probe and probe inserting parameters, which will be helpful to reduce tissue injuries induced by probe insertion and thus improve the working life of neural probe to meet the long-term clinical application.
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<p><b>OBJECTIVE</b>To observe effects of Tanshinone- II A sulfonate on expression of Nuclear factor-kappaB (NF-kappaB), Vascular Cell Adhesion Molecule-1 (VCAM-1) and hemorrheology during spinal cord ischemia reperfusion injury,and explore the function and mechnism.</p><p><b>METHODS</b>Fifty-four New Zealand rabbits (aged 3 months,weighted 2.0 +/- 0.2 kg) were randomly divided into 6 in sham group (lumbar artery were separated in operation,0.8 ml/kg saline were injected at 0.5 h before and after operation), 24 in ischemia group ( lumbar artery were clipped after seperation, and the same dose of saline), 24 in Tanshinone group (lumbar artery were clipped after seperation, and the same dose of Tanshinone- II A sulfonate) . Abdomincal aorta blood were drawed after treatment respectively at 0.5 h, 1 h, 4 h and 8 h, and tesetd whole blood viscosity [high cut (mpa.s)/150(l/s), middle cut (mpa.s)/60(l/s) and low cut (mpa.s)/10(l/s)], capillary plasma viscosity, red cell aggregation index, rigid index, deformation index and electrophoresis index. Spinal cord tissues were divided into two sections,one fixed in 4% paraformaldehyde, another stored in liquid nitrogen. Immunohistochemical method and ELISA were used to test change of content of NF-kappaB and VCAM-1.</p><p><b>RESULTS</b>1) The expression of NF-kappaB in Tanshinone group were lowest, and in ischemia group were highest. 2) Compared with sham group, VCAM-1 in ischemia group at different time were obviously increased,especially at 0.5, 1 and 4 h (P<0.01), and had meaning at 8 h (P<0.05). Compare between Tanshinone group and ischemia group, VCAM-1 at 0.5 h were obviously decreased (P<0.01), and had meaning at 1 h, 4 h and 8 h (P<0.05). 3) There were no postive vasvular expression in sham group, and at 0.5 h in Tanshinone group and ischemia group. The highest postive vasvular expression in ischemia group were at 1 h, 4 h and 8 h, and had significant meaning at 1 h and 4 h between ischemia group and Tanshinone group (P<0.05), and 8 h were obviously most. 4) The whole blood viscosity in ischemia group at 10 s(-1), 60 s(-1), 150 s(-1) were highest, and capillary viscosity increased (P<0.05 or P<0.01). While capillary viscosity, red cell aggregation index, figid index, deformation index in Tanshinone group decreased obviously (P<0.01).</p><p><b>CONCLUSION</b>Tanshinone-II A sulfonate can relieve spinal cord ischemia reperfusion injury by regulating expression of NF-kappaB, VCAM-1, decreasing whole blood viscosity, capillary plasma viscosity, red cell aggregation index, rigid index, and improve hemorhelogy.</p>
Subject(s)
Animals , Female , Male , Rabbits , Gene Expression Regulation , Hemorheology , NF-kappa B , Metabolism , Phenanthrenes , Pharmacology , Therapeutic Uses , Reperfusion Injury , Drug Therapy , Metabolism , Spinal Cord , Metabolism , Vascular Cell Adhesion Molecule-1 , MetabolismABSTRACT
Objective To improve the long-term biocompatibility and mechanical stability of Titanium alloy implants. Method micro-nano patterned TiO2 thin films have been prepared on TC4 substrates via a sol-gel dip-coating method with Ti(OC4H9)4 (TEOT) as precursor and polyethylene glycol (PEG) as organic template. The influence of PEG concentration upon the morphology of the target films has been studied by using AFM. The wettability and tribological properties of the target films have been studied by means of optical contact angle measuring instrument and UMT-2. Results When increasing the PEG concentration to 3.5g/100mL, a parallel arranged groove structure can be obtained on the surface of the sample; compare with TiO2 sol-gel film without adding organic template, the wear resistance of the patterned film is better. Conclusions The feature of the patterned TiO2 films can be controlled by adding different amount of organic template; and it has been proved that a certain kind of patterned structure can improve the wear resistance of the film.
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Objective To improve the long-term biocompatibility and mechanical stability of Titanium alloy implants.Method Micro-nano patterned TiO_2 thin films have been prepared on TC4 substrates via a sol-gel dipcoating method with Ti(OC_4H_9)_4(TEOT)as precursor and polyethylene glycol(PEG)as organic template.The Influence of PEG concentration upon the morphology of the target films has been studied by using AFM.The wettability and tribological properties of the target films have been studied by means of optical contact angle measuring instrument and UMT-2.Results When increasing the PEG concentration to 3.5 g/100 mL,a parallel arranged groove structure can be obtained on the surface of the sample;compared with TiO_2 sol-gel film without adding organic template,the wear resistance of the patterned film is better.Conclusions The feature of the patterned TiO_2 films can be controlled by adding different amount of Organic template;and it has been proved that a certain kind of patterned structure can imeprove the wear resistance of the film.
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Objective To improve the long-term biocompatibility and mechanical stability of Titanium alloy implants.Method Micro-nano patterned TiO_2 thin films have been prepared on TC4 substrates via a sol-gel dipcoating method with Ti(OC_4H_9)_4(TEOT)as precursor and polyethylene glycol(PEG)as organic template.The Influence of PEG concentration upon the morphology of the target films has been studied by using AFM.The wettability and tribological properties of the target films have been studied by means of optical contact angle measuring instrument and UMT-2.Results When increasing the PEG concentration to 3.5 g/100 mL,a parallel arranged groove structure can be obtained on the surface of the sample;compared with TiO_2 sol-gel film without adding organic template,the wear resistance of the patterned film is better.Conclusions The feature of the patterned TiO_2 films can be controlled by adding different amount of Organic template;and it has been proved that a certain kind of patterned structure can imeprove the wear resistance of the film.
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<p><b>OBJECTIVE</b>To observe and survey the location of Xiaguan (ST 7), "Die'e" and Quanliao (SI 18) on the surface, and the needling depth and direction from the 3 points to sphenopalatine ganglion.</p><p><b>METHODS</b>Fifteen corpses (30 sides) of adult male were fixed by 10% formalin. The lateral areas of face were dissected from the surface to the deep on the 3 acupoints: the electric drill with the kirschner wire punctured towards the sphenopalatine ganglion and extended to the contralateral areas according to different directions of puncturing sphenopalatine ganglion from the 3 acupoints. The corresponding puncturing points of the 3 acupoints were measured by the coordinate location method.</p><p><b>RESULTS</b>(1) Surface location: the distance between Quanliao (SI 18) and "Die'e" was 21 mm and the distance between Xiaguan (ST 7) and "Die'e" was 17 mm; (2) Inserting depth of each point to sphenopalatine ganglion: the depths of Xiaguan (ST 7), "Die'e" and Quanliao (SI 18) were 49.9 mm, 46.9 mm and 46.6 mm, respectively; (3) The coordinate location of the corresponding puncturing points: the puncturing direction of Xiaguan (ST 7) was anterointernal upper corresponding to the area of connecting center between contralateral Taiyang (EX-HN 5) and Tongziliao (GB 1), the distance between the corresponding inserting point of Xiaguan (ST 7) and Sizhukong (TE 23) was 17.6 mm; the puncturing direction of "Die'e" point was posterointernal upper, and the horizontal distance from the corresponding puncture point to the zygomatic arch was 33 mm and the vertical distance from the corresponding puncture point to the eyes' outer canthus was 42 mm; the puncturing direction of Quanliao (SI 18) was posteriointernal upper and the distance between the corresponding inserting point and the area of contralateral parietal tuber, the distance between the corresponding inserting point of Quanliao (SI 18) and the connecting line of bilateral external acoustic pore was 28 mm, the distance between the corresponding inserting point of Quan-liao (SI 18) and the medial line of the head was 62 mm.</p><p><b>CONCLUSION</b>Understanding the surface location, inserting depths and the general puncturing directions of the 3 points can provide basis for puncturing the sphenopalatine ganglion in clinical practice.</p>
Subject(s)
Adult , Humans , Male , Acupuncture Points , Cadaver , Electroacupuncture , Methods , Face , Ganglia, Parasympathetic , Physiology , Palate , Sphenoid SinusABSTRACT
<p><b>OBJECTIVE</b>To identify the genetic alterations in nonsyndromic cleft lip and palate (NSCLP).</p><p><b>METHODS</b>Comparative genomic hybridization was applied to investigate the genomic imbalance (the gain or loss of genetic material) in 7 cases of NSCLP.</p><p><b>RESULTS</b>It showed that the loss of chromosome DNA copies happened in chromosome 6, 7, 10, 13, 14, 16, 20, 22 and the gain of chromosome DNA copies happened in chromosome 5, 15, 18, 19. Conclusions 13q had a high frequency (71.4%) of chromosome loss.</p><p><b>CONCLUSIONS</b>Abnormal chromosome DNA copies happen in all the patients with NSCLP. Most of the patients have chromosome DNA copies loss. It suggests that loss of inhibitory gene may be related to the NSCLP. The related inhibitory gene may locate in 13q.</p>
Subject(s)
Adolescent , Adult , Child , Child, Preschool , Humans , Infant , Young Adult , China , Ethnology , Cleft Lip , Genetics , Cleft Palate , Genetics , Comparative Genomic Hybridization , DNA , Genetic Variation , Genotype , Mutation , PhenotypeABSTRACT
<p><b>OBJECTIVE</b>To study on the relation between the regional anatomy and safety of acupuncture at Tiantu (CV 22) and Qishe (ST 11).</p><p><b>METHODS</b>In forty-six adult corpses, 92 sides were dissected to observe the partial anatomy structure of acupuncture path of Tiantu (CV 22) and Qishe (ST 11) and structure characteristics by the steel needle marked method and lay-by-lay dissection method.</p><p><b>RESULTS</b>The bilateral the pleura returning lines behind the manubrium sterni interacted at the sternal angle plane accounted for 50.0% of the total specimens and at the first ribs plane accounted for 6.5% of the specimens; for needling the point Tiantu (CV 22), left brachiocephalic vein was at the same level or close to the manubrium sterni upper fringe in 43.5% of the specimens, the left brachiocephalic vein and the middle of manubrium sterni were at the same level in 56.5% of the specimens; for needling the point Qishe (ST 11), in 68 sides of the specimens, internal jugular vein were pierced, accounting for 73.9%, and in 24 sides of the specimens the left common carotid artery were pierced, accounting for 26.1%; in 50 sides of specimens the vagus nerve were touched by the steel needle, accounting for 54.3.</p><p><b>CONCLUSION</b>In acupuncture of Tiantu (CV 22) and Qishe (ST 11), the needle not only easily injure the upper pleural cavity, but also damage the big blood vessel and the vagus nerve in the mediastinum and the cervical root.</p>
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Adult , Aged , Female , Humans , Male , Middle Aged , Acupuncture Points , Acupuncture Therapy , Methods , Manubrium , Pleura , SafetyABSTRACT
<p><b>OBJECTIVE</b>To explore the relation of the form of pleural cupula of the normal adult with safety of acupuncture at commonly-used acupoints around the pleural cupula.</p><p><b>METHODS</b>The safe depth for the commonly-used acupoints around the pleural cupula and the relation with the form of pleural cupula were investigated in 46 adult corpses with small Kirschner wire location and arrangement dissection.</p><p><b>RESULTS</b>The width of the pleural cupula projection equal to clavicle medial 1/3 accounted for 32. 6% of all the corpses, and the width of the pleural cupula projection more than clavicle medial 1/3 accounted for 59. 8% of all the corpses, the width of the pleural cupula projection less than clavicle medial 1/3 and pleural cupula medial margin located at the sternoclavicular joint medial accounted for 7.6% of all the corpses. The observed points such as Tiantu (CV 22), Qishe (ST 11), Jianjing (GB 21), Dingchuan (EX-B1), Dazhu (BL 11) which were considered be not related to the pleural cupula. When acupuncture is carried out according to criteria of acupoint location and needling direction, and the needle exceeded a limit, the pleural menbrane will be broken and induce destruction.</p><p><b>CONCLUSION</b>Position and form of the pleural cupula have anatomical relation to acupuncture accident for needling the points around the superior pleural cupula, which should be played attention to.</p>
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Adult , Female , Humans , Male , Acupuncture Points , PleuraABSTRACT
<p><b>OBJECTIVE</b>To study the association of TGF-alpha gene Taq I polymorphism and nonsyndromic cleft lip with or without cleft palate (NSCL/P) in Chinese.</p><p><b>METHODS</b>107 patients with NSCL/P and 136 healthy controls were examined for TGF-alpha/Taq I genotypes. TGF-alpha/Taq I typing was carried out by digesting the locus specific polymerase chain reaction amplified products with alleles specific Taq I restriction enzyme (PCR-RELP).</p><p><b>RESULTS</b>The C2 allele frequency of TGF-alpha/Taq I in patients with NSCL/P (16%) was significantly higher than that in healthy controls (8%). The C2 genotype frequency of TGF-alpha/Taq I in NSCL/P patients with positive family history (12.5%) was significantly higher than that in healthy controls.</p><p><b>CONCLUSION</b>These findings demonstrate the role of TGF-alpha as a gene of major effects in the development of nonsyndromic cleft lip with or without cleft palate clefts in human. These findings suggest that a family history of clefting may correlate with the TGF-alpha Taq I rare variation.</p>